提取出的线粒体可以应用于SDS-PAGE，immunoblottings,ELISA,IP,膜蛋白结构分析，2-D，酶活性检测和其他应用
Figure 1. Enrichment of Mitochondrial Marker (Ubiquinol-Cytochrome C Reductase Core Protein) with MinuteTM Mitochondria Isolation Kit.
A. SDS-PAGE profiles of total protein extract vs. isolated mitochondrial proteins. Lanes 1, 3 5, total proteins from isolated mouse kidney cells, mouse skeletal muscle and liver tissues respectively. Lanes 2, 4, 6, isolated mitochondrial proteins from mouse kidney cells, mouse skeletal muscle and liver tissues respectively.
B. Western blotting. Proteins in A were transferred to nitrocellulose membranes and probed with anti-ubiquinol-cytochrome C reductase core protein (ab96333, abcam, Cambridge, MA) and anti-lamin B1 (ab16048,a nuclear envelope marker protein. abcam, Cambridge, MA).
C. Densitometry measurement of mitochondrial marker signals.
| Minute TM Mitochondrial Membrane Protein Isolation Kit (50 Tests) | MP-007 | 4327.00 |
| Minute TM Mitochondrial Membrane Protein Isolation Kit (4 Tests) | MP-007 | 436.00 |
简单，快速，性价比高
得率高，可获得更完整的线粒体
处理样品范围更广
缓冲液系统不含表面活性剂和EDTA
1. Gou, R. et al. (2014). Resveratrol suppresses oxidized low-density lipoprotein-induced macrophage apoptosis through inhibition of intracellular reactive oxygen species generation, lox-1 and the p38 MAPK pathway. Cellular Physiology and Biochemistry. 34:603-616. DOI: 10.1159/000363026
2. Hongli Hu, et al (2016).Acetylationof PGK1 Promotes Liver Cancer Cell Proliferation and Tumorigenesis. Hepatology.doi:10.1002/hep.28887
